当它们被创建时，螺旋上没有核苷酸。要添加核苷酸，请使用函数draw_strand(id, antisense, begin, end, color)，其中

• id是螺旋标识符
• antisense是一个布尔值，表示我们在螺旋的哪个轴上绘制（false表示义轴，true表示反义轴）
• begin和end是添加核苷酸的轴上的第一个和最后一个（包含）坐标
• color是要绘制的链的颜色。可以使用十六进制RGB指定颜色，或者当感到缺乏灵感时使用AUTO_COLOR。

use codenano::*;


pub fn main() {
    let mut ori = Nanostructure::new();
    let id_0 = ori.add_grid_helix(0, 0);

    let id_1 = ori.add_helix(0., 0.2, 0., 0., 0., 0.); 
    // ^ This helix is parallel to id_0

    let id_2 = ori.add_helix(0., 0.5, -1., 0., 3.14/4., 3.14/6.);
    // ^ This helix has different orientation

    ori.draw_strand(id_0, false, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_0, true, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_1, false, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_1, true, 0, 20, AUTO_COLOR);

    // green strand
    ori.draw_strand(id_2, false, 0, 40, 0x00FF00);

    // blue strand
    ori.draw_strand(id_2, true, 0, 40, 0x0000FF);

    ori.finish();
}

跳跃

链确定核苷酸之间的共价键的位置。如果两个相邻的核苷酸在同一个链上，它们之间就有共价键。也可以通过在两个核苷酸之间创建一个jump来创建一个共价键。从链s1上的一个核苷酸跳跃到链s2上的另一个核苷酸有以下效果

• s1的5'端和s2的3'端合并为一个链
• s1的3'端成为一个新的独立链
• s2的5'端成为一个新的独立链

use codenano::*;

pub fn main() {
    let mut ori = Nanostructure::new();
    let id_0 = ori.add_grid_helix(0, 0);

    let id_1 = ori.add_helix(0., 0.2, 0., 0., 0., 0.); 
    // ^ This helix is parallel to id_0

    ori.draw_strand(id_0, false, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_0, true, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_1, false, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_1, true, 0, 20, AUTO_COLOR);

    ori.make_jump(ori.get_nucl(id_0, 11, false), ori.get_nucl(id_1, 11, false));
    ori.finish();
}

示例

双重交叉

use codenano::*;


pub fn main() {
    let colors: Vec<u32> =vec![0x1f1f1f, 0xf81118,0xecba0f,0x2a84d2,
                               0x4e5ab7, 0xd6dbe5, 0x1dd361, 0x0f7ddb];


    let mut ori = Nanostructure::new();
    let id_0 = ori.add_grid_helix(0, 0);
    let id_1 = ori.add_grid_helix(1, 0);

    ori.draw_strand(id_0, false, 0, 20, colors[6]);
    ori.draw_strand(id_0, true, 0, 20, colors[1]);
    ori.draw_strand(id_1, false, 0, 20, colors[2]);
    ori.draw_strand(id_1, true, 0, 20, colors[3]);

    ori.make_jump(ori.get_nucl(id_1, 11, false), ori.get_nucl(id_0, 11, true));
    ori.make_jump(ori.get_nucl(id_0, 12, true), ori.get_nucl(id_1, 12, false));


    ori.finish();
}

单链瓷砖

use codenano::*;

const tile_length:isize = 11; 



static mut colornum:usize = 0;

pub fn add_sst(ori: &mut Nanostructure, helix_id: usize, helix_pos: isize) {
        let colors: Vec<u32> =vec![0x1f1f1f, 0xf81118,0xecba0f,0x2a84d2,
                                   0x4e5ab7, 0xd6dbe5, 0x1dd361, 0x0f7ddb];
        let mut color = 0;
        unsafe {
        color = colors[ (1 * colornum) % colors.len()];
        colornum += 1; }
        ori.draw_strand(helix_id, false, helix_pos, helix_pos + tile_length, color);
        ori.draw_strand(helix_id + 1, true, helix_pos + tile_length, helix_pos, color);
        ori.make_jump(ori.get_nucl(helix_id, helix_pos +tile_length, false),
                      ori.get_nucl(helix_id + 1, helix_pos + tile_length, true));
}


pub fn main() {

    let mut ori = Nanostructure::new();
    let id_0 = ori.add_grid_helix(0, 0);
    let id_1 = ori.add_grid_helix(1, 0);
    let id_2 = ori.add_grid_helix(2, 0);
    let id_3 = ori.add_grid_helix(3, 0);
    let id_4 = ori.add_grid_helix(4, 0);
    let id_5 = ori.add_grid_helix(5, 0);
    let id_6 = ori.add_grid_helix(6, 0);

    for i in 0isize..5 {
        for j in 0usize..3 {
            add_sst(&mut ori, j * 2, i * tile_length + i);
            add_sst(&mut ori, j * 2 + 1, i * tile_length + i + tile_length/2);
        }
    }
    ori.finish();
}

高级用户功能

更改常量

有两个常量可以更改。第一个是每转的碱基对数，第二个是两个反向对之间的角度。

默认情况下，每转的碱基对数是10.4，两个反向对之间的角度是220度（而不是180度，这就是为什么有主/次沟槽）。要更改这些，必须创建一个DNAConst对象，修改其值，并将其作为Nanostructure对象构造函数的参数传递。

use codenano::*;


pub fn main() {

    let mut cst = DNAConst::default();
    cst.set_bpp(10.7); // number of base pair per turn
    cst.set_groove(3.14); // pi angle => no minor/major groove
    let mut ori = Nanostructure::with_constant(cst);
    let id_0 = ori.add_grid_helix(0, 0);
    let id_1 = ori.add_grid_helix(1, 0);

    ori.draw_strand(id_0, false, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_0, true, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_1, false, 0, 20, AUTO_COLOR);
    ori.draw_strand(id_1, true, 0, 20, AUTO_COLOR);

    ori.make_jump(ori.get_nucl(id_1, 11, false), ori.get_nucl(id_0, 11, true));
    ori.make_jump(ori.get_nucl(id_0, 12, true), ori.get_nucl(id_1, 12, false));


    ori.finish();
}

许可

Codenano在MIT许可和Apache许可（版本2.0）的条款下分发